UAE UNIVERSITY
FACULTY OF SCIENCE
DEPARTMENT OF BIOLOGY
Course Outline
Course Title :Practical
Cell Biology and Genetics
Course Number : Biol
373
Prerequisite : Biol
105
Laboratory Instructor : Sherif El-Alfy
Laboratory Coordinator : Dr Amr Amin
Text Book : Peter J. and Russel (1994;
2000).
Fundemental of Genetics. Harpor
Collins.
Credit Hours : 1 Hr.
Semester : Fall
2001/2002
Course
objectives:
1.
To provide an in-depth approach toward understanding
fundamental key aspects of Cell Biology and Genetics, including structural and
functional features of cell components and organelles as well as the pattern of
inheritance involved in different traits.
2.
To provide an understanding of the problems
regarding principles and procedures of identifying underlying genetics basis of
traits in higher plants and animals including man.
3.
To develop an understanding of the techniques used
in Genetics and Cell Biology and how to utilize these techniques in empirical
investigation of biological phenomena.
4.
To provide experience in the interpretation of
experimental data and to enhance oral presentation.
Skills to be
gained:
Upon the completion of this course, the
student should be able to
1.
Be familiar with a wide range of experiments.
2.
Verify the Mendelian concept of
dominance-recessiveness, segregation and independent assortment through
experimental crosses.
3.
Apply statistical tests to genetic data.
4.
Identify and interpret the different stages of
mitosis and meiosis.
5.
Use computer-based learning materials to extend
their knowledge of genetic and cellular techniques and their application.
6.
Read primary text and write a summary report.
Goals and course outcome
1.
To provide an in-depth approach toward understanding
fundamental key aspects of cell Biology and Genetics,
including structural and functional features of cell components and organelles
as well as the pattern of inheritance involved in different traits.
·
Apply genetic and cell techniques to
biological analysis including plant, animal, and microbial systems
·
Apply genetic and cell biology techniques
and integrate into theoretical aspects in order to understand the underlying
phenomenon
·
Explain the fundamental of genetics and
cell biology experiments in a clear, comprehensive and balanced manner
·
Demonstrate the differences and similarities
between cellular and genetics functions of eukaryotes and prokaryotes
·
Demonstrate the difference and
similarities between plant and animal genetics
2.
To provide an understanding of the problems
regarding principles and procedures of identifying underlying genetics basis of
traits in higher plants and animals including man.
· Explain
and correlate between molecular, Mendelian, and population genetics
· Illustrate
experiments involving different genetic and cellular levels
3.
To develop an understanding of the techniques used
in Genetics and Cell Biology and how to utilize these techniques in empirical
investigation of biological phenomena.
·
Transform the experiments into
laboratory reports(5)
·
Collect and tabulate data
systematically from experiments (6)
·
Analyze genetic and cell biology data
from various sources and integrate them into the experiments(4)
4.
To provide experience in the interpretation of
experimental data and to enhance oral presentation.
·
Demonstrate the experiments with
regarding to understanding genetic and cell biology concepts
·
Explain the relevance the experiments
to the application of genetic and cell biology
·
Employ genetic and cellular biology
experiments to basic and applied sciences(3)
·
Explain the advantages and
disadvantages of experiments
Teaching aids:
1. Board 2.
Overhead transparencies 3. Video films
4. CD 5.
Photos 6. Models
7. Slides 8. Preserved
specimens 9. Internet
Grading:
Quizzes 20%
Activities 20%
Midterm 20%
Final Exam 40%
Course
Contents:
|
Lab 1 9/2/2002 |
Microscopy and cytological studies - The compound microscope - Wet mount preparation |
|
Lab 2 16/2/2002 |
Cell fractionation -
Determination of the centrifugation speed required
for chloroplast isolation -
Isolation and examination of chloroplast fraction -
Determination of the sedimentation coefficient -
Isolation and examination of nuclear and
mitochondrial fraction |
|
Lab 3 23/2/2002 |
Membrane permeability -
Membrane structure -
Plasmolysis and deplasmolysis -
Penetration of nonelectrolytes |
|
Lab 4 2/3/2002 |
Mitosis -
The cell cycle -
Mitosis squash technique in plants -
Mitosis in animal cells |
|
Lab 5 9/3/2002 |
Meiosis -
Meiotic stages -
Study of microsporogenesis using squash technique
in maize |
Lab 6
|
Drosophila culture
for genetic experiments - Background (media, insect development, insect handling, sex differentiation and virgin collection) - Some Drosophila mutations - Balancer chromosomes - Monohybrid crosses - Test crosses
|
|
Lab 7 23/3/2002 |
Drosophila continuation
- Dihybrid crosses - Reciprocal
crosses |
|
Lab 8 30/3/2002 |
Midterm Exam
|
|
Lab 9 6/4/2002 |
Drosophila continuation - Salivary gland
chromosomes - Dissection of Drosophila
larvae - Preparation of
Polytene chromosomes |
|
Lab 10 13/4/2002 |
The effect of genes on the expression of other genes
( gene interaction ) - Maize genetics - Chi square
test (X2) |
|
Lab 11 20/4/2002 |
Human karyotype and phenotypes - Chromosome morphology -
Normal and abnormal Karyotype -
Human phenotypes |
|
Lab 12 27/4/2002 |
Detection of gene frequency in human population - Calculation of gene frequency -
Blood grouping
|
|
Lab 13 4/5/2002 |
Isolation of DNA from an animal cell -DNA isolation
from liver cells and / or blood. |
|
Lab 14 11/5/2002 |
Restriction endonuclease analysis - DNA digestion -
Agarose gel electrophoresis -
PCR |
|
Las 15 18/5/2002 |
Protein electrophoresis and sequencing - Polyacrylamide gel electrophoresis -
Reading sequencing autoradiogram |
|
Lab 16 25/5/2002 |
Final Exam
|
