test 2

UAE UNIVERSITY

FACULTY OF SCIENCE

DEPARTMENT OF BIOLOGY

COURSE OUTLINE SPRING 2000

BIOLOGICAL TECHNIQUES AND INSTRUMENTATION

COURSE NUMBER BIOL468

Prepared by Dr. Amr Amin

Prerequisite: Animal Biology BIOL210

Credit hours: 1 hour (3 hrs laboratory sessions)

Books and References:

Skoog DA and Leary JJ. 1992. Principles of Instrumental Analysis. Saunders College Publishing.

Interesting Web Sites:

http://www.scimedia.com/chem-ed/analytic/ac-meths.htm

http://www.scimedia.com/chem-ed/scidex.htm

http://www.scimedia.com/index.htm

Course Objectives:

1- To develop the ability to deal with basic laboratory instruments and to introduce basic experimental techniques.

2- To understand the theory of basic instruments and practical applications of basic techniques in order to provide students with knowledge that conciliate the job market

3- Enhance students' ability for self-learning by using problem based learning and problem solving.

Learning Skills:

1. Students should be able to understand the theoritical setting and practical application of most laboratory equipments that are used in biological analysis.

2. Students should be able to manipulate available equipments to their research needs.

Teaching Philosophy:

· Curriculum should match the needs and interest of young scientists and provide them with essential concepts in biological techniques and instrumentation

· Students relate these concepts to problems that encounter in their daily lives and improve their prospects for lifelong education.

· Constant changes in biology make it difficult to define interactions among most biological systems; however, principal theory and relation between subsequent changes should be taught. Contemporary facts should also be presented to students as they are published.

· Concept of Scientific Thinking and relationship to new discoveries in science should be presented.

· All available audiovisual materials should be adequately used.

Student Evaluation:

1. Weekly quizzes and evaluation 20 points

2. Continuous evaluation of practical skills 10 points

3. Midterm examination 20 points

(Section 51,. April , 2000)

(Section 52,. April , 2000)

(Section 01,. April , 2000)

4. Final laboratory project 20 points

5. Final examination 30 points

(Section 51,. June , 2000)

(Section 52,. June , 2000)

(Section 01,. June , 2000)

Teaching Activities:

Both lectures and laboratories will be complementary to each other

Term Planning (Spring 2001)

WK #	Week	Lecture	Field trips
1	Feb., 17	Biosafty and Introducing main equipments in the Dept. of Biology
2	Feb., 24	Solution, buffers and pH measurements
3	Mar., 3	Spectrophotometery, visible
4	Mar., 10	Spectrophotometery, ultraviolet, fluorometry
5	Mar., 17	Seperation and purification techniques, Chromatography, HPLC
6	Mar., 24	Seperation and purification techniques, Chromatography, GLC, TLC, Ion exchange	trip to Centeral Laboratory Unit
7	Mar., 31	Seperation and purification techniques, Electrophoresis, agarose gel	trip to Centeral Laboratory Unit
8	Apr., 7	Seperation and purification techniques, Electrophoresis, polyacrylamide gel.
9	Apr., 14	Midterm
10	Apr., 21	Radiobiological techniques
11	Apr., 28	RIA and ELISA	Trip to Tawam Hospital
12	May, 5	Electron Microscopy	Trip to Faculty of Medicin
13	May., 12	Microscopes	Trip to Faculty of Medicin
14	May., 19	Tissue culture equipments, hoods, and safty procedure
15	May., 26	Assessement of cell culture, DNA and protein quantitation
16	June 2	Final exam